Jurnal Pendidikan Biologi Undiksha

Pengembangan Media Pembelajaran Video Animasi Interaktif Menggunakan Aplikasi Powtoon pada Materi Bioteknologi Kelas XII

Syafri Ramadhan — 2024-09-06

Packet books, LKS (student worksheets) and whiteboards are still learning media that are often used, while the percentage from preliminary study results shows that 96.8% of students are more interested and enthusiastic if interactive animated videos are used in the learning process. This research aims to determine the design, feasibility and practicality of developing interactive animated video learning media. This research uses Research and Development research using the ADDIE (Analyze, Design, Development, Implementation, Evaluation) model. This research used rating scale calculations for feasibility testing by one media expert and one material expert and Likert scale calculations for practicality testing by one biology teacher and 15 students. The media expert feasibility test results, if calculated in percentage terms, get a score of 93.75% which is categorized as very feasible. The results of the material expert feasibility test, if calculated in percentage terms, get a score of 84.38% which is categorized as very feasible. The results of the teacher practicality test, if calculated in percentage terms, get a score of 96.67% which is categorized as very practical. The results of the students' practicality test, if calculated in percentage terms, get a score of 90.10% which is categorized as very practical. So, it can be said that the development of interactive animated video learning media using the Powtoon application in class XII biotechnology material that has been developed is very feasible and very practical when used in the learning process.

Perbedaan Profil Protein Plasma Darah Mencit Menggunakan SDS-PAGE Tanpa Penambahan dan dengan Penambahan 2-Mercaptoethanol

Khaerunissa Anbar Istiadi — 2024-09-06

SDS-PAGE is a method used to analyze proteins based on differences in molecular weight. Sample preparation in SDS-PAGE with 2-mercaptoethanol will reduce disulfide bonds in proteins. The sulfide bond reduction process will cause differences in protein conformation and profile. The purpose of this study was to compare the pattern of blood plasma protein profiles of mice analyzed using SDS-PAGE, with the addition of 2-mercaptoethanol and without the addition of 2-mercaptoethanol in a mixture of sample buffer solutions. The results of SDS-PAGE showed that the blood plasma protein profile of mice without the addition and with the addition of 2-mercaptoethanol showed differences in the number of protein bands, the molecular weight of several proteins and the thickness of the protein bands. There were nine protein bands with a molecular weight above 52 kDa in the profile without the addition of 2-mercaptoethanol, while there were only five protein bands with a molecular weight above 52 kDa in the profile with the addition of 2-mercaptoethanol. The difference in protein profile patterns shows that mouse blood plasma contains proteins containing disulfide bonds that can be reduced by 2-mercaptoethanol.

Isolasi dan Identifikasi Kandungan Metabolit Sekunder Jamur Endofit Rimpang Kunyit (Curcuma longa linn.)

Dewi Chusniasih — 2024-09-06

Turmeric is a plant that is often used as an ingredient in traditional medicine. Turmeric rhizome contains secondary metabolites such as alkaloids, triterpenoids, flavonoids, tannins, anthraquinones and polyphenols which have the potential to be used as antimicrobials and antioxidants. Endophytic fungi that live in plant tissue can produce the same metabolites as their host plants, so that endophytic fungi from turmeric rhizomes have the potential to produce secondary metabolites of medicinal ingredients. This research aimed to isolate endophytic fungi from turmeric rhizomes, and identify secondary metabolites produced by these endophytic fungi. Endophytic fungal isolates were carried out using the pour plate method, and purification was carried out on each colony which had different characteristics. Identification of secondary metabolites was carried out qualitatively. The results showed that there were 5 isolates of endophytic fungi from turmeric rhizomes, namely isolates J1, J2, J3, J4, and J5. The five isolates of endophytic fungi were positive for containing alkaloids, tannins and phenolics, but only isolates J2, J3 and J4 also produced saponins. The five isolates of endophytic fungi were reported not to produce flavonoid compounds. Secondary metabolites produced by endophytic fungi from turmeric rhizomes have the potential to be used as antimicrobial and antioxidant natural ingredients.

Analisis Minat Belajar Siswa Kelas X-1 di SMAN 1 Kramatwatu pada Pembelajaran Biologi Materi Virus

Annisa Hidayah — 2024-10-16

This research aims to analyze the learning interests of class X-1 students related to learning virus material in biology subjects. The method used in this research is a qualitative descriptive approach with data collection techniques in the form of questionnaires and observations. The data obtained was analyzed using scoring to reveal a clearer picture of students' interest in learning. The results of the research showed that students in class X-1 at SMAN 1 Kramatwatu showed high interest in studying virus material in the biology subject. This is shown by the enthusiastic and responsive attitude of students during learning activities. Students also seem focused and pay attention to the lesson so that the material can be easily understood. Students' interest in learning is influenced by several factors. Factors that influence students' interest in learning can be divided into two categories: internal factors, such as learning style and interest in learning, and external factors, including learning models, learning methods, and material studies. These factors are mutually involved in forming students' interest in learning about viruses in biology.

Remediasi Kromium Heksavalen Melalui Bioaugmentasi Konsorsium Bakteri Indigenous Wilayah Tercemar Limbah Cair Sablon dengan Biostimulasi Bulu Ayam Broiler

Made Luhur Sahadeva — 2024-10-16

Hexavalent chromium or Cr (VI) is a heavy metal that is dangerous to living things if it enters the body's metabolism because of its high permeability and oxidation ability which can disrupt biological systems. Cr (VI) is found in large quantities in textile dyes in the screen printing industry. The results of washing screen printing produce Cr (VI) waste which is dangerous to the environment. The waters that are the place where the waste from washing screen printing flows become polluted and toxic due to waste that is not treated first. One way to overcome this pollution is by bioremediation. In this research, bioremediation was carried out using a consortium of indigenous bacteria with biostimulation of broiler chicken feathers. The bacterial consortium was taken from sediments of waters contaminated with Cr (VI) waste, then isolated and tested for tolerance to chromium. The bacterial consortium that is resistant to chromium was then used for bioremediation by a combination of bioaugmentation and biostimulation using broiler chicken feathers as a nutrient supply for the bacteria. This research aims to obtain a bacterial consortium with the highest effectiveness of Cr (VI) remediation through a combination of biaugmentation and biostimulation of broiler chicken feathers. Base on results of isolation and tolerance tests show that indigenous bacteria from polluted areas have the ability to survive and grow even in conditions contaminated with chromium. However, the addition of nutrients from broiler chicken feather meal as a carbon and nitrogen source did not succeed in increasing the metabolic activity of bacteria in degrading Cr(VI). The results of the ANOVA one way test also support the conclusion that there is no significant difference in effectiveness between treatments with varying concentrations of chicken feather flour. Bacterial consortia have not been able to improve Cr (VI) remediation due to nutritional incompatibility and difficulties in decomposing nutrients in chicken feathers, as well as the possibility of antagonistic interactions between microbial strains.

Pembelajaran yang Repetitif Meningkatkan Keluhan Musculoskeletal dan Stres Akibat Belajar serta Menurunkan Motivasi Siswa di SMA Negeri 10 Denpasar

I Made Heri Gunawan — 2024-10-16

This study aims to determine the application of repetitive learning increases musculoskeletal complaints and stress due to learning and decreases learning motivation. This type of research is a quasi-experimental study with a nonequivalent randomized pre and post test control group design. The dependent variables of this study include: (1) musculoskeletal complaints were recorded using the Nordic Body Map questionnaire, (2) stress due to learning was recorded using the stress questionnaire due to learning, (3) learning motivation was recorded using the learning motivation questionnaire. Data were analyzed using an independent sample t-test with a significance level of 5%. The results of this study indicate that (1) repetitive learning significantly increases musculoskeletal complaints in students by 27.95%, (2) repetitive learning significantly increases stress due to learning by 13.79%, (3) repetitive learning significantly decreases learning motivation by 3.36%.